B-131 The Prevalence and Clinical Relevance of the DFS Immunofluorescent Staining Pattern in a Large ANA-positive Cohort

Abstract Background The dense fine-speckled (DFS) nuclear pattern is one of the most common indirect immunofluorescence (IIF) patterns detected during routine antinuclear antibody (ANA) screening. Although various researchers have studied DFS in recent years, it presents a clinical puzzle since its significance remains elusive. Anti-DFS-70 antibodies occur healthy individuals with different medical conditions. Unlike other ANA patterns, they are not associated systemic autoimmune disease. present study examined association conditions patients who presented to tertiary care center. Methods Retrospective chart review all laboratory values (January 2020 through June 2022) ANA- and DFS-positive tests ordered by clinicians compared patient diagnosis reported. UMass Human IRB approves this study. Results Of 8885 samples analyzed, 67.8% (6022) were reported negative. total positive (2863) samples, 78.6% (2257) had 9.1% (260) mitotic 12.1% (346) cytoplasmic patterns. 14.6% (417) For pattern, titers ranged from 1:40 1:1280; 62.5% between1:40, 1:80. for 76% (317) female patients, 90% (375) >21 years age. Among commonly diseases neurological, followed respiratory, digestive, circulatory, musculoskeletal (Table 1). Conclusion Clinicians should use indicate pathological phenomena than diseases. Only 3.1% rheumatic disorder. pay close attention Patterns, especially staining when using testing.